Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.

نویسندگان

  • J A Higgins
  • M C Jenkins
  • D R Shelton
  • R Fayer
  • J S Karns
چکیده

The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 67 11  شماره 

صفحات  -

تاریخ انتشار 2001